Clone Read LE_HBa0197I09_SP6_110600

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0197I09_SP6_110600
Primer
SP6
ID from Seq. Facility
LE_HBa_197_I09-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
519 trimmed for vector and quality, 867 raw
Genbank Accession
DU096051
Sequence
>LE_HBa0197I09_SP6_110600 (raw untrimmed)
TTTTTTTAACTCTCTCTTCCTATATACTTAGAAGGATGATGCCCGCGCATTGCACGGGCCCAACGTGTCATCCCAATAAATAAATTATTAAGAATTGTGT
ATACAACATTTGTATCCTATTTTTACAATATGTGCAAAATTAATGCTAGGTAAAATTTAATTGAGATGGTTGTCAATCCTTCTATTTTGCAAAAATGAAA
TTCAAGTAAATTTTTTCAAAACATTTGTATAAAAAGAAAAAAGTTGCAAATCATATCATCGTTAACCACTTCCTTGTGGTTTCGTGGAGATATCAATGTA
GACATTACGGAAAACGTGGTAGCATATGTCCCTGTAATCACAGCTAAAAAGGCATAGCATGAATAATTATTTGTACCTGAGATAACAAATACAGTTTTTG
AGCCCTCAAGCATGATGGCTAATGTAATCCAAAGGCATTGAAAATAGCGAAAATTGCGCGGCTAAGCAAACTTATACTATTTTATTACTCATCATAATTA
TAGTTTGCTATAGTTATCACTTGCGACTAAAACTATACACTAATTATGTGGGCTGACTTCCAGTTTTTTATATTAGTCATGTCTGTATATATATAATTCG
GTAGGATATACAAATGCTTATGTATAAATATACAATTATTTAACTTATATACATATACAATTCACCCTCTCCCCCCTTATATCCCCTTTCCTCGCTTGTC
TTCTCTCTCCCCTTCTCCCATTCTAGTTTGCCTCATTCCCCCCCTCTCCCAAACCTCACTATGCCCATAATACACATATTTATGTTGATAAAATAACACA
AGTAAAATACTCACTTCCAAATTAACTCTCTGTTCCAACCTCGTCAGTTCCCCGCTCACAGCAGAGC
>LE_HBa0197I09_SP6_110600 (trimmed for quality and cloning vector)
TGATGCCCGCGCATTGCACGGGCCCAACGTGTCATCCCAATAAATAAATTATTAAGAATTGTGTATACAACATTTGTATCCTATTTTTACAATATGTGCA
AAATTAATGCTAGGTAAAATTTAATTGAGATGGTTGTCAATCCTTCTATTTTGCAAAAATGAAATTCAAGTAAATTTTTTCAAAACATTTGTATAAAAAG
AAAAAAGTTGCAAATCATATCATCGTTAACCACTTCCTTGTGGTTTCGTGGAGATATCAATGTAGACATTACGGAAAACGTGGTAGCATATGTCCCTGTA
ATCACAGCTAAAAAGGCATAGCATGAATAATTATTTGTACCTGAGATAACAAATACAGTTTTTGAGCCCTCAAGCATGATGGCTAATGTAATCCAAAGGC
ATTGAAAATAGCGAAAATTGCGCGGCTAAGCAAACTTATACTATTTTATTACTCATCATAATTATAGTTTGCTATAGTTATCACTTGCGACTAAAACTAT
ACACTAATTATGTGGGCTG

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.944512
Expected error %
0.38738
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g19780.1 Score 75 E Value 3e-14 Identity 24.20% Align. Length 197bp (37.96%) Frame 2
68418.m02351 tubulin alpha-3/alpha-5 chain (TUA5) nearly identical to SP|P20363 Tubulin alpha-3/alpha-5 chain {Arabidopsis thaliana}
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g19770.1 Score 75 E Value 3e-14 Identity 24.20% Align. Length 197bp (37.96%) Frame 2
68418.m02350 tubulin alpha-3/alpha-5 chain (TUA3) nearly identical to SP|P20363 Tubulin alpha-3/alpha-5 chain {Arabidopsis thaliana}
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA66075.1| Score 76 E Value 4e-13 Identity 24.20% Align. Length 197bp (37.96%) Frame 2
alpha-tubulin [Avena sativa] sp|Q38771|TBA_AVESA Tubulin alpha chain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAD20821.1| Score 76 E Value 4e-13 Identity 24.20% Align. Length 197bp (37.96%) Frame 2
alpha tubulin [Zea mays]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0197I09
Clone
Clone name
hba-197i9
LE_HBa0197I09
P197I09
Clone type
BAC
Contamination
none
Estimated length
116,542
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found