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Clone Read LE_HBa0167D14_T7_12843

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0167D14_T7_12843
Primer
T7
ID from Seq. Facility
LE_HBa_167_D14-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
795 trimmed for vector and quality, 840 raw
Genbank Accession
DU031514
Sequence
>LE_HBa0167D14_T7_12843 (raw untrimmed)
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGAAGACCATCCCACAGCAGCCTTAGGTAACAAAAAGATATATACACAATTC
CAGCTGAAAATCATAGTGTGAAGGCATAAATGCCCGAAATGAACCTCAACTCAGCATTCAAAAGAAGTGAAATTGATTGCTAGTAGACAATAATGTCTCC
TTCATGTGGTCACATGTACTATATAATTATGAACCAGCACAACATATGTGGTTAAATAGTATAAGTTGAATTACCCTTTCCGTTTCAAGTAAATAGGTCG
AATTGTTTCTTCACTTTTTCAAAACAATCAGGTTAGACATATGAGTAGTTTTAAAGAATTCATTTACAAATACCACAAGAAAAAGAAAATGTTAATATGA
CTCCTACTGCCCCTACCATTTGCCCTCCAACTTTAACAAACGTAATCTTGACTGAAGAAAGAAAATAATATGTCACGACTCAAGAAACTATAACTATTTG
CAAAAACAGGAGAACTTACCCAAAAGAGTCATCAAAGACGAAAGGAAGCATCCCAGCGCGGTTATTATCTGTATCGGTCCAAATGTGGCTGGTGAAACAA
ACAAAAAATTGTTATGTACAACTTCGATTATAGTCCTCAAAGAAACAAGCATTATATTTTTTAAACACATACCTTCTCTTGCTGAGATAACCATTAGGTT
TTCCTTCAGTGAAAGGTGAATTTGCAAATAGAGCTGTAGCAATCTGTTTCATTGTATTTAGTTTAAGATAACTAAATAAGTTTCTTGTAAAAATAGAAAG
GAAAGAAAGGATAAAGGTACCTTACAGGCTGCAAGGCAAG
>LE_HBa0167D14_T7_12843 (trimmed for quality and cloning vector)
AGCTTTGAAGACCATCCCACAGCAGCCTTAGGTAACAAAAAGATATATACACAATTCCAGCTGAAAATCATAGTGTGAAGGCATAAATGCCCGAAATGAA
CCTCAACTCAGCATTCAAAAGAAGTGAAATTGATTGCTAGTAGACAATAATGTCTCCTTCATGTGGTCACATGTACTATATAATTATGAACCAGCACAAC
ATATGTGGTTAAATAGTATAAGTTGAATTACCCTTTCCGTTTCAAGTAAATAGGTCGAATTGTTTCTTCACTTTTTCAAAACAATCAGGTTAGACATATG
AGTAGTTTTAAAGAATTCATTTACAAATACCACAAGAAAAAGAAAATGTTAATATGACTCCTACTGCCCCTACCATTTGCCCTCCAACTTTAACAAACGT
AATCTTGACTGAAGAAAGAAAATAATATGTCACGACTCAAGAAACTATAACTATTTGCAAAAACAGGAGAACTTACCCAAAAGAGTCATCAAAGACGAAA
GGAAGCATCCCAGCGCGGTTATTATCTGTATCGGTCCAAATGTGGCTGGTGAAACAAACAAAAAATTGTTATGTACAACTTCGATTATAGTCCTCAAAGA
AACAAGCATTATATTTTTTAAACACATACCTTCTCTTGCTGAGATAACCATTAGGTTTTCCTTCAGTGAAAGGTGAATTTGCAAATAGAGCTGTAGCAAT
CTGTTTCATTGTATTTAGTTTAAGATAACTAAATAAGTTTCTTGTAAAAATAGAAAGGAAAGAAAGGATAAAGGTACCTTACAGGCTGCAAGGCA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.94648
Expected error %
1.11382
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23100.1 Score 79 E Value 2e-15 Identity 16.50% Align. Length 290bp (36.48%) Frame 3
68417.m03329 glutamate-cysteine ligase / gamma-glutamylcysteine synthetase (GSH1) identical to glutamate-cysteine ligase SP:P46309 from [Arabidopsis thaliana]; contains Pfam profile: PF04107 glutamate-cysteine ligase family 2(GCS2)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAB71230.1| Score 89 E Value 1e-16 Identity 18.00% Align. Length 293bp (36.86%) Frame 3
gamma-glutamylcysteine synthetase [Lycopersicon esculentum] pir||T04332 glutamate-cysteine ligase (EC 6.3.2.2) - tomato sp|O22493|GSH1_LYCES Glutamate--cysteine ligase, chloroplast precursor (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD27390.1| Score 81 E Value 4e-14 Identity 16.70% Align. Length 293bp (36.86%) Frame 3
gamma-glutamylcysteine synthetase [Zinnia elegans]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0167D14
Clone
Clone name
hba-167d14
LE_HBa0167D14
C08HBa0167D14
P167D14
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found