Clone Read LE_HBa0025I06_SP6_135935

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0025I06_SP6_135935
Primer
SP6
ID from Seq. Facility
LE_HBa_025_I06-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
642 trimmed for vector and quality, 954 raw
Genbank Accession
DU045081
Sequence
>LE_HBa0025I06_SP6_135935 (raw untrimmed)
ACGGTTTTGCTACAAGGGGATAATAGATAGTAGAAGATCGTTCTTTTTATTCCAGAGTTCAAATACAATCTTTTGTCAGTATCACGATTGACAAAACAAC
TCAACTGCGATGTGGTATTTTTCCCTGACTTTTGTGTATTTTAGGATCTCTTCAATGGGAGGGTCCTGGGGATTGGTAAAGAAGATCAAGGTCTATATTT
GCTTACTTCAGACATCAAACCAAGATCAGTTTCTACTGCTGCATGCTCTTTCTCATGTAATAGTCAAAATAGCAATTTTCATAACGCCCATAGTTCCACT
AGTTTTACTTCCTCTTTGTCTACAAGTGTATGTTCGACTGGACTTTGGCATAAGAGACTGGGACATGCCCCTCTGAAAGTTCTTAATAGAATAAAGTGTA
CTCATAATATCTCTTTGAAAGATTTTAGTTGTATTGTATGTCCACTTGCTAAACAGCCAATGTTACCATTTCTTCATAGTTTGTCTACATCTGAGTCTAT
GATATTGTACATGCTGATGTATGGGGTTCTTACAGAGTTCAAAATTATGATGGCAAGAGGTATTTCCTTACTTTGATAGATGATTTATCCAGATACACTT
GGATTTTTTTAATGCACAATAAGGCTGATTCCTTGTTATACTGAAGAATTTTGTGTGTATGATAAAAACTCAGTTTGGGGTAAGTATAGAATGTGTGAGG
TCAGATATTGTTCAGAGTTATTAATTCCTCAAGTACCTGATTGTTCAAGGATATGGAATAAAACAACAAATTTTTGTGTTTACTCTCCTCACAAAATATT
GTTGTGAATGAAACACAGGCTATTCTGAAAGGTTCGATCTTTGAGTTCCAGCTGTGTTCCTTTAAATTTTGGGGTTCCGGGCCATACCAATTTTTTATAA
ATAAACGCCCCTGGGGTTTACAGTTTCTTCCTTTAAAAATTTTGGGCCCCCCAC
>LE_HBa0025I06_SP6_135935 (trimmed for quality and cloning vector)
AGTTCAAATACAATCTTTTGTCAGTATCACGATTGACAAAACAACTCAACTGCGATGTGGTATTTTTCCCTGACTTTTGTGTATTTTAGGATCTCTTCAA
TGGGAGGGTCCTGGGGATTGGTAAAGAAGATCAAGGTCTATATTTGCTTACTTCAGACATCAAACCAAGATCAGTTTCTACTGCTGCATGCTCTTTCTCA
TGTAATAGTCAAAATAGCAATTTTCATAACGCCCATAGTTCCACTAGTTTTACTTCCTCTTTGTCTACAAGTGTATGTTCGACTGGACTTTGGCATAAGA
GACTGGGACATGCCCCTCTGAAAGTTCTTAATAGAATAAAGTGTACTCATAATATCTCTTTGAAAGATTTTAGTTGTATTGTATGTCCACTTGCTAAACA
GCCAATGTTACCATTTCTTCATAGTTTGTCTACATCTGAGTCTATGATATTGTACATGCTGATGTATGGGGTTCTTACAGAGTTCAAAATTATGATGGCA
AGAGGTATTTCCTTACTTTGATAGATGATTTATCCAGATACACTTGGATTTTTTTAATGCACAATAAGGCTGATTCCTTGTTATACTGAAGAATTTTGTG
TGTATGATAAAAACTCAGTTTGGGGTAAGTATAGAATGTGTG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.950196
Expected error %
0.2106
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU89728.1| Score 71 E Value 2e-26 Identity 34.70% Align. Length 633bp (98.60%) Frame 1
putative retroelement pol polyprotein-like [Solanum tuberosum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAB61111.1| Score 68 E Value 4e-20 Identity 27.30% Align. Length 577bp (89.88%) Frame 1
Strong similarity to Zea mays retrotransposon Hopscotch polyprotein (gb|U12626). [Arabidopsis thaliana] pir||G96722 hypothetical protein F20P5.25 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0025I06
Clone
Clone name
hba-25i6
LE_HBa0025I06
C01HBa0025I06
P025I06
Clone type
BAC
Contamination
none
Estimated length
95,663
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found