Clone Read LE_HBa0005K05_T7_2025

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0005K05_T7_2025
Primer
T7
ID from Seq. Facility
LE_HBa_005_K05-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
802 trimmed for vector and quality, 931 raw
Genbank Accession
DU022275
Sequence
>LE_HBa0005K05_T7_2025 (raw untrimmed)
TTCGGACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGCCTACATAAAGCCACATTCCAATTCCCACATCCTAATATAATTCAAACTAAC
ATGTGCATTATCTTCACCGAGTATACCAATGGTACTTCCCTTTTTTCCTCAGTTCCACCCCAGGAGTAGTATCAACATTTCCTATCCACCTCCTTCGCAA
CATTCTGAGGAAGAATGAACACTGATCTACAAAAATTATGAATTGAAAACGAGATAGCCAGAACCACTTAGAGCCTCCTTCCATAAGAGAGTTGTTATGC
ATAAAACATTTTATATCATATTTGTTATTTTATCAACTAGTTAATTGGTGACATTCGAGTTTACCCCACCTCTTAGATGTGAGTGAGAGTACAAAATATC
TAATGAGAAAAAAAACCTTTGTGTAAATTCAGTAATCTTCAAGAGTTGCTTCTTCATTTGTTTATTCATCCCTGCTAAGAAAAGGCTGGATTTTTTAATG
TTTGCCACAAGTCCAGTAATAACACTAAAGTGAACTAATGATTTCATGACCCTGGTAACAGATTTGACATCTCCTTTACTAAAGATCATTTGCAAAGACA
AGATGGTTCAGTCTAAAAGTTTTACACATAGGGTGAAATTGCAAATCTGGTAGATAGGTCATGCTATTGAGCAGCCTTGAGAGGTACTCCATAAATAGTA
CAAAGAGTAGAGGGGAGAGAGGGTCTCATTGTCTAAATCCTCTCTTTCCATCGAAACAACCATAACCTTCTCCATAGTGAACTTTGTTGTTGATACACAT
ACCAGTATTCATTTAATGAATCTTTCTGAAATTCAAATCCTCTTAGAGATTTGTCAATTTTCTGTTTGAATGTCTCTCAAAGGTGAAAAGTCTTTCCATT
TATATAGTGGAAGAATTAAAAGAATTACCCA
>LE_HBa0005K05_T7_2025 (trimmed for quality and cloning vector)
AGCTTGCCTACATAAAGCCACATTCCAATTCCCACATCCTAATATAATTCAAACTAACATGTGCATTATCTTCACCGAGTATACCAATGGTACTTCCCTT
TTTTCCTCAGTTCCACCCCAGGAGTAGTATCAACATTTCCTATCCACCTCCTTCGCAACATTCTGAGGAAGAATGAACACTGATCTACAAAAATTATGAA
TTGAAAACGAGATAGCCAGAACCACTTAGAGCCTCCTTCCATAAGAGAGTTGTTATGCATAAAACATTTTATATCATATTTGTTATTTTATCAACTAGTT
AATTGGTGACATTCGAGTTTACCCCACCTCTTAGATGTGAGTGAGAGTACAAAATATCTAATGAGAAAAAAAACCTTTGTGTAAATTCAGTAATCTTCAA
GAGTTGCTTCTTCATTTGTTTATTCATCCCTGCTAAGAAAAGGCTGGATTTTTTAATGTTTGCCACAAGTCCAGTAATAACACTAAAGTGAACTAATGAT
TTCATGACCCTGGTAACAGATTTGACATCTCCTTTACTAAAGATCATTTGCAAAGACAAGATGGTTCAGTCTAAAAGTTTTACACATAGGGTGAAATTGC
AAATCTGGTAGATAGGTCATGCTATTGAGCAGCCTTGAGAGGTACTCCATAAATAGTACAAAGAGTAGAGGGGAGAGAGGGTCTCATTGTCTAAATCCTC
TCTTTCCATCGAAACAACCATAACCTTCTCCATAGTGAACTTTGTTGTTGATACACATACCAGTATTCATTTAATGAATCTTTCTGAAATTCAAATCCTC
TT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.947955
Expected error %
0.167574
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAB09269.1| Score 66 E Value 2e-11 Identity 48.40% Align. Length 294bp (36.66%) Frame 3
unnamed protein product [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAD21699.1| Score 58 E Value 5e-11 Identity 42.50% Align. Length 339bp (42.27%) Frame 3
Contains reverse transcriptase domain (rvt) PF|00078. [Arabidopsis thaliana] pir||F86436 hypothetical protein F28K20.4 - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0005K05
Clone
Clone name
hba-5k5
LE_HBa0005K05
P005K05
Clone type
BAC
Contamination
none
Estimated length
67,584
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found