Clone Read LE_HBa0089B11_SP6_25028

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0089B11_SP6_25028
Primer
SP6
ID from Seq. Facility
LE_HBa_089_B11-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
450 trimmed for vector and quality, 938 raw
Sequence
>LE_HBa0089B11_SP6_25028 (raw untrimmed)
TCGGGGCTGATTTCCCCCAAGGCTTGTACAATGGTGCTAGAACTCTCTTCAATTTTCTAGAGACCAGCGTGGAAGATCCATTGTCGGCAATGAAGCTGAA
ACTTTCTATAATTCCACCGGTTATTGGGATGAGTTTATATGGGGTGCGGCTTGGCTGTATTATGCTACTGGAAATTCTTCATATCTTCAGCTTGCTACAA
CACCTGGTATTGCCAAACATGCTGGTGCTTTCTGGGGAGGTCCTGATAGGGGTGCTCGCGGGAGGCGTGCTCAGCAGGGATAACAAGCTACGAGAGGCGC
AGCATACACAAACCTCACCTCTTCTTCCTTACCCTCCTCATATTCTGTGCACTTTGTGCTTGAACTTAAACNCANANNTATTCTAAGCTAGGATATCTCA
TTTCTTCGAGGGACTTATTTTTTGCTATAACACTGCTTTTTGTTATCTCTTCAGGTGCTACTGAGCCGTATGAGGCTGTTTCTGAGCCCTGGATACCCTT
AGAAAGAAATTTTAAGGACATTTCATAACCAGAACAGCATAATCAGGCCCCCGCACTTCTTTTTTCCCCAANCGTTTGGGTGTGTCCGTAAAAGGAAACC
TTTCATTACCCAATTCGCGTCAAAGTAATTTGAAAACTTTAGTTTGAAAATCAAAACAAAATCCTTTTGCCCTTTCCATCAGGGGCAATGCGATTTAAAC
AATTTTAAAATGTATTACCTTTACCAACTTGCTCTCAATTAGAGTTATGCATCCTCCGGCTCCTGTTAATGACTCTTCACTTCCAACCTCTTAAAGTATT
GTGACAATTATCTATCAGATTTCTTTCTTATTATCATTCCATTAATGAATTTTTTACAATTTCCCTCTTTTAATTTCATTCCCTTCCAGTCCTAAAATCT
TCGCTTTCCAGTCTTTAATTTTTACCCCGGGTGAGTGT
>LE_HBa0089B11_SP6_25028 (trimmed for quality and cloning vector)
GTGCTAGAACTCTCTTCAATTTTCTAGAGACCAGCGTGGAAGATCCATTGTCGGCAATGAAGCTGAAACTTTCTATAATTCCACCGGTTATTGGGATGAG
TTTATATGGGGTGCGGCTTGGCTGTATTATGCTACTGGAAATTCTTCATATCTTCAGCTTGCTACAACACCTGGTATTGCCAAACATGCTGGTGCTTTCT
GGGGAGGTCCTGATAGGGGTGCTCGCGGGAGGCGTGCTCAGCAGGGATAACAAGCTACGAGAGGCGCAGCATACACAAACCTCACCTCTTCTTCCTTACC
CTCCTCATATTCTGTGCACTTTGTGCTTGAACTTAAACNCANANNTATTCTAAGCTAGGATATCTCATTTCTTCGAGGGACTTATTTTTTGCTATAACAC
TGCTTTTTGTTATCTCTTCAGGTGCTACTGAGCCGTATGAGGCTGTTTCT

This is probably not a useful sequence.

Problems:
  • High predicted error rate
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.994664
Expected error %
4.1256
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAC49704.1| Score 140 E Value 1e-32 Identity 95.50% Align. Length 252bp (56.00%) Frame 3
endo-1,4-beta-glucanase [Lycopersicon esculentum] pir||T07612 cellulase (EC 3.2.1.4) Cel3, membrane-anchored - tomato
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAS45400.1| Score 122 E Value 3e-27 Identity 83.30% Align. Length 252bp (56.00%) Frame 3
endo-1,4-beta-glucanase [Populus tremuloides]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0089B11
Clone
Clone name
hba-89b11
LE_HBa0089B11
P089B11
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found