Clone Read LE_HBa0089B11_T7_25029

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0089B11_T7_25029
Primer
T7
ID from Seq. Facility
LE_HBa_089_B11-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
765 trimmed for vector and quality, 918 raw
Genbank Accession
DU039888
Sequence
>LE_HBa0089B11_T7_25029 (raw untrimmed)
CGACCCCGGGTCCCGGGGAATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGTGCTGGAGATCCAGATATGCCATATAGATGTTTGCGTTGCTGATCTC
ACCTCAAGTAAGTTACAAGCTAAGACGCGGTTCTAATAATATCTATACGTAATTAATTATCTTACAAGTGATCTGATTGTGAAACAGCTTTGAAATTAAA
CGGATTTACATGCAAGAACATGTTCTCCGCGGCTGATTTCTCTTCGATGGTTATTTCAAAGCCAGGAGCCACAAACAACATGTTTGGTTCCCTTGTTACT
GGAGCTAACGTTATGGCTATTCCTGGGCTTAACACCTTAGGTGTGTCCATGGCTCGAATCGACTATGCTCCAGGTGGCATTAACCCACCCCACCTCCACC
CACGAGCCACGGAGATGATTTACGTTTTACAAGGTGAATTAGATGTTGGTTTTATTACTACAGCTAATGTATTGGTCTCTAAGCATATTGTACAAGGAGA
AGTGTTTGTTTTTCCAAGAGGACTTGTTCATTTTCAGAAGAATAATGGTCACATGCCAGCAGCTGTTATTGCTGGGTTCAATAGTCAGTTAGCTGGTACA
CAGTCGATTGCGACAACGTTGTTTGCAGCAACACCAGCACCTGGTGTTCCTAATGATATCTTGGCTCAAGCATTCCAAATTGGTACCATGCAAGTTCAGC
AAATTAAGGCTAAATTTGCACCCAGGAATTAAGTAGTAAAATCTTTATTTCTCAACAGATTTGTTTTAATTCTGGTTGTTCTGTTTGACAAGTGGATGAT
GTTTTGATTTTTGGTTGAAATGTTAATTAGAGAAAATTCTCCTTAAAATTCTCATTATGTGTTTGGTGTTACAATATATATGTTGGGAAATTGGATTTTG
AGTGGTTTGTGTTCGAAT
>LE_HBa0089B11_T7_25029 (trimmed for quality and cloning vector)
AGCTTGTGCTGGAGATCCAGATATGCCATATAGATGTTTGCGTTGCTGATCTCACCTCAAGTAAGTTACAAGCTAAGACGCGGTTCTAATAATATCTATA
CGTAATTAATTATCTTACAAGTGATCTGATTGTGAAACAGCTTTGAAATTAAACGGATTTACATGCAAGAACATGTTCTCCGCGGCTGATTTCTCTTCGA
TGGTTATTTCAAAGCCAGGAGCCACAAACAACATGTTTGGTTCCCTTGTTACTGGAGCTAACGTTATGGCTATTCCTGGGCTTAACACCTTAGGTGTGTC
CATGGCTCGAATCGACTATGCTCCAGGTGGCATTAACCCACCCCACCTCCACCCACGAGCCACGGAGATGATTTACGTTTTACAAGGTGAATTAGATGTT
GGTTTTATTACTACAGCTAATGTATTGGTCTCTAAGCATATTGTACAAGGAGAAGTGTTTGTTTTTCCAAGAGGACTTGTTCATTTTCAGAAGAATAATG
GTCACATGCCAGCAGCTGTTATTGCTGGGTTCAATAGTCAGTTAGCTGGTACACAGTCGATTGCGACAACGTTGTTTGCAGCAACACCAGCACCTGGTGT
TCCTAATGATATCTTGGCTCAAGCATTCCAAATTGGTACCATGCAAGTTCAGCAAATTAAGGCTAAATTTGCACCCAGGAATTAAGTAGTAAAATCTTTA
TTTCTCAACAGATTTGTTTTAATTCTGGTTGTTCTGTTTGACAAGTGGATGATGTTTTGATTTTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.97812
Expected error %
1.2064
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g09560.1 Score 244 E Value 4e-65 Identity 21.30% Align. Length 554bp (72.42%) Frame 3
68414.m01072 germin-like protein (GLP4) (GLP5) identical to Arabidopsis germin-like protein subfamily 2 member 1 [SP|P94014]; Location of EST 180L10T7, gi|906417
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g62020.1 Score 235 E Value 2e-62 Identity 21.40% Align. Length 554bp (72.42%) Frame 3
68416.m06966 germin-like protein (GLP10) identical to germin-like protein subfamily 2 member 4 [SP|Q9M263]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||T04361 Score 362 E Value 6e-99 Identity 27.90% Align. Length 680bp (88.89%) Frame 3
probable germin protein - tomato dbj|BAA25197.1| germin-like protein [Lycopersicon esculentum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAR97545.1| Score 279 E Value 2e-74 Identity 71.90% Align. Length 646bp (84.44%) Frame 1
germin-like protein [Nicotiana attenuata]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0089B11
Clone
Clone name
hba-89b11
LE_HBa0089B11
P089B11
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found