Clone Read LE_HBa0089B22_T7_25051

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0089B22_T7_25051
Primer
T7
ID from Seq. Facility
LE_HBa_089_B22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
736 trimmed for vector and quality, 884 raw
Genbank Accession
DU039910
Sequence
>LE_HBa0089B22_T7_25051 (raw untrimmed)
GTGTGGCCGGGACCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTAGCTTAAGAAGACGCGGGACGTTTTAAAAGATTAAACTTCTTGAA
AATTACTAAACCTTTAATCATTTCCTTTTTTATCAGTTTCAAAAAGAATATCACATTTACTTATATCGTAAGTATTTAAAGATATAATTCTTTTTACCCT
TATCGATTCCACTTAATTTTAAAGATAAGTATTCTAATACATTTATGAGGTAACAGAAAAGTTAATTACTCTCTTCGTTTAAAAAAGAATTACCTTGTTT
TTTTTGGTATGTTTAAAAAAGAATAACCACTTTTCTTTTTTTACAAGACTTTAACTTTAGCTATCCACGTGACATGTTTAAGACCACAAAATTAAAAGGT
ATTTTGATACACTTGACATAACTTTAAATTACAACCACAATATTAAAAAGTATTCTTTCTTTTCTTAAACTCCGTTCCAAGTCAAACGAAACCAATCTTT
TTGAAACGAATGGAAGTATTTTTTTAAGAGTAATTTGGTAAACATTTCAAAGTTTTTATTATTTCTTAAATTTCGTGTCCGATCAAATGTTGTCACATAA
AATGAGAGGAGGAAATACTCAAGCGACTTCAATAAGGGCAATTTGGTAAATATAATAAAATCTTCTATTATTTTTTTAACTTCATATTCAATCAAACAAA
CAATGTCACATGAAAAAACTCTAATCAAATTAGAAATGCCCCTCATGAACAACAACATGCAACTTCAGATAATTTAGTCGATCCCTAAACAGATGCCCGT
AACGAAAACCCCAAATTGGGAATGTTGTTCGTGAACTCCAATAGATAGAAAAATATCAAAAGCAAAAGTAGTTTAGCTTCCCAT
>LE_HBa0089B22_T7_25051 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTTAGCTTAAGAAGACGCGGGACGTTTTAAAAGATTAAACTTCTTGAAAATTACTAAACCTTTAATCATTTC
CTTTTTTATCAGTTTCAAAAAGAATATCACATTTACTTATATCGTAAGTATTTAAAGATATAATTCTTTTTACCCTTATCGATTCCACTTAATTTTAAAG
ATAAGTATTCTAATACATTTATGAGGTAACAGAAAAGTTAATTACTCTCTTCGTTTAAAAAAGAATTACCTTGTTTTTTTTGGTATGTTTAAAAAAGAAT
AACCACTTTTCTTTTTTTACAAGACTTTAACTTTAGCTATCCACGTGACATGTTTAAGACCACAAAATTAAAAGGTATTTTGATACACTTGACATAACTT
TAAATTACAACCACAATATTAAAAAGTATTCTTTCTTTTCTTAAACTCCGTTCCAAGTCAAACGAAACCAATCTTTTTGAAACGAATGGAAGTATTTTTT
TAAGAGTAATTTGGTAAACATTTCAAAGTTTTTATTATTTCTTAAATTTCGTGTCCGATCAAATGTTGTCACATAAAATGAGAGGAGGAAATACTCAAGC
GACTTCAATAAGGGCAATTTGGTAAATATAATAAAATCTTCTATTATTTTTTTAACTTCATATTCAATCAAACAAACAATGTCACATGAAAAAACTCTAA
TCAAATTAGAAATGCCCCTCATGAACAACAACATGC

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.911352
Expected error %
0.0752922
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M19092.1:1-90 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.08%) Frame 2
Cloning vector pJRD179 DNA
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.08%) Frame 2
Cloning vector pMAMneoBlue
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0089B22
Clone
Clone name
hba-89b22
LE_HBa0089B22
C08HBa0089B22
P089B22
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found