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Clone Read LE_HBa0090G19_T7_26053

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0090G19_T7_26053
Primer
T7
ID from Seq. Facility
LE_HBa_090_G19-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
778 trimmed for vector and quality, 921 raw
Sequence
>LE_HBa0090G19_T7_26053 (raw untrimmed)
GTGGAAGGTGGGTCCGGGGGGTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTTCTTGTTGCGAACATAAGTTGCAGAATCCTTCCTATCCCCAAC
AAGTATAACTGCAAGGCCAGGAACAACACCAATCGAATCTTTCATCCTAGTTATTTCAGCATTTATTTCATCTCTAACATCTTTTGCAACTTTTTTCCCA
TCAATCACTTTTACCAATGCCTCATTAGCCATCGCAGCTGCACAGCAAGGCGAATAATTCAAAAATTCTTAAAAGGTAGGTCTGTCAAACCAAACCAACA
ATGCCACAAAACCAGACAGTTGGGGAACACGCAAGAAAAAATCCAGAGTGAAACAATTACATTAAAAGGAAAAAGTCCCAATTGCGAGCCAAGGACCAAA
CACTTCAAGTCCACGAAATTCTCAACCAACTTACTTAAGAATACTGAAGTTCAGAAAATTGTAGACATATCACTCTGACCAATTTAACAGCTTACAGAGA
TGGTTTTTATAGTAAAGCTCTGAACTTTTAATACCTATACCATCAATTAATCCCATTTTAGATCTCTGAATCCTTGTTTGGTCATCTTCTAGCTAAAGAT
TCTTAACATCACGCCTAAAACAGCTTGCCAATATTATAACTTTACTCAAAACATAGATTTTATGTATTGTCAATTACTCAATATCTAGTTCATTCGCCAA
AAAAAAATATACTACCTTTCCCCTTTTTTAAATCCATATTCCATACAAGATATACTTACAAAGCAACTTAAAAGCTTGGGAATTGCAAGAAAAAGAATTT
CCCGGGTGAGATGGAGGGAGGAGGAAAATTTGGAAAAGGGATGATGGTTTTGGGAAATTATGGTTTGTAAAGGTACCCCTCAAGTACGTGAACAAAATCT
TGGGGTTCCTAGAAAAAGTTA
>LE_HBa0090G19_T7_26053 (trimmed for quality and cloning vector)
AGCTTTTTTCTTGTTGCGAACATAAGTTGCAGAATCCTTCCTATCCCCAACAAGTATAACTGCAAGGCCAGGAACAACACCAATCGAATCTTTCATCCTA
GTTATTTCAGCATTTATTTCATCTCTAACATCTTTTGCAACTTTTTTCCCATCAATCACTTTTACCAATGCCTCATTAGCCATCGCAGCTGCACAGCAAG
GCGAATAATTCAAAAATTCTTAAAAGGTAGGTCTGTCAAACCAAACCAACAATGCCACAAAACCAGACAGTTGGGGAACACGCAAGAAAAAATCCAGAGT
GAAACAATTACATTAAAAGGAAAAAGTCCCAATTGCGAGCCAAGGACCAAACACTTCAAGTCCACGAAATTCTCAACCAACTTACTTAAGAATACTGAAG
TTCAGAAAATTGTAGACATATCACTCTGACCAATTTAACAGCTTACAGAGATGGTTTTTATAGTAAAGCTCTGAACTTTTAATACCTATACCATCAATTA
ATCCCATTTTAGATCTCTGAATCCTTGTTTGGTCATCTTCTAGCTAAAGATTCTTAACATCACGCCTAAAACAGCTTGCCAATATTATAACTTTACTCAA
AACATAGATTTTATGTATTGTCAATTACTCAATATCTAGTTCATTCGCCAAAAAAAAATATACTACCTTTCCCCTTTTTTAAATCCATATTCCATACAAG
ATATACTTACAAAGCAACTTAAAAGCTTGGGAATTGCAAGAAAAAGAATTTCCCGGGTGAGATGGAGGGAGGAGGAAA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.94669
Expected error %
1.13205
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g00620.1 Score 91 E Value 7e-19 Identity 27.70% Align. Length 158bp (20.31%) Frame 2
68417.m00086 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g38660.1 Score 67 E Value 8e-12 Identity 15.30% Align. Length 248bp (31.88%) Frame 2
68415.m04748 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB80871.1| Score 91 E Value 3e-17 Identity 27.70% Align. Length 158bp (20.31%) Frame 2
putative tetrahydrofolate synthase [Arabidopsis thaliana] gb|AAN15385.1| Unknown protein [Arabidopsis thaliana] gb|AAL24426.1| Unknown protein [Arabidopsis thaliana] gb|AAC13627.1| F6N23.26 gene product [Arabidopsis thaliana] ref|NP_191971.1| tetrahydrofolate dehydrogenase/cyclohydrolase, putative [Arabidopsis thaliana] pir||T01226 probable methylenetetrahydrofolate dehydrogenase (NADP) (EC 1.5.1.5) F6N23.26 - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_450549.1| Score 71 E Value 4e-11 Identity 16.90% Align. Length 200bp (25.71%) Frame 2
methylenetetrahydrofolate dehydrogenase-like [Oryza sativa (japonica cultivar-group)] dbj|BAD23599.1| methylenetetrahydrofolate dehydrogenase-like [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0090G19
Clone
Clone name
hba-90g19
LE_HBa0090G19
P090G19
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found