Clone Read LE_HBa0140G03_SP6_260883

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0140G03_SP6_260883
Primer
SP6
ID from Seq. Facility
LE_HBa_140_G03-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
653 trimmed for vector and quality, 986 raw
Genbank Accession
DU082521
Sequence
>LE_HBa0140G03_SP6_260883 (raw untrimmed)
NNNCCCATGAGGGGATCTCTTTGTATTCCATTGGAGAAGAAAGCAAAGTAGGATAACTGATGAAATAAATAAATAGAACAGCATCCCCTTTGATCAATCA
AAACACTAATGATTTAGAAAACTAGCACTGACTATACACATTTGCAAGATTGCCAGAATCTAACTTCTTGTGCATCCAATCAATGATATCCTGGGCAATT
TCTTCACGCTCAGGCTCAAATAGAAGATCGTGCAAGAATCCATCATAAAGTTTAATGTCTTTGAATTCAGAAGCAGCCTCATTGTACAAATCTTGAGAAG
CCAATGGATCAGTCACTCTATCAGCAGATCCATGGAGGACTAAGAATGGGACAGTGACTGACTTGAAGTTCCGCATCAAGTAAGACGAGATACGCAGAAT
CTCATGCCCTGTCCTCACCCTCAAAGGCCCAGTATACACTAGAGGGTCAGAATACTTAGCCACCAGTGCGGCAGGATCCCTTGAAACAGGGATTCCCCTT
TTGTGAGAACCTTTGAACTGGTACCTAGGTGCAACCAATGAAAAAATTGGTGCCACAGCCTGCATTTCGATAACAAACACAAAGTTAATGAAAGAAGACA
CTGCTGTGAAAACACATTCATCAAAGCACACGAAAATAACCATATAAGGGTTAGGCGCCTTACACTAACAATAAGATTTGCAGGTTTCACACGCAATGCT
GGTGAAGTCAATATGATGCCCCTCCACCCTGTTTTCAATATGTGGAAAAGAAGCTGCCTGTGCAAGGGAACCAAATAATCAATGAAAATCTTTAGGGTTT
CAAAATGGTCCAAAGCAAATTTCACTTCAAAGCCCTTTCCTTTAAAACCCCCCCTCCTCCCGTTTGAATGACCAAAGAGGAAACCTTGGTATCCCAGGGT
TTTGTAAACTTGAACTTTTTTCAAAAAAACCCCCCCGTATTAAAATTTCAAAAGAAATTTTATGGGGTTAAATGTGCCCGGCCCTC
>LE_HBa0140G03_SP6_260883 (trimmed for quality and cloning vector)
TCTTTGTATTCCATTGGAGAAGAAAGCAAAGTAGGATAACTGATGAAATAAATAAATAGAACAGCATCCCCTTTGATCAATCAAAACACTAATGATTTAG
AAAACTAGCACTGACTATACACATTTGCAAGATTGCCAGAATCTAACTTCTTGTGCATCCAATCAATGATATCCTGGGCAATTTCTTCACGCTCAGGCTC
AAATAGAAGATCGTGCAAGAATCCATCATAAAGTTTAATGTCTTTGAATTCAGAAGCAGCCTCATTGTACAAATCTTGAGAAGCCAATGGATCAGTCACT
CTATCAGCAGATCCATGGAGGACTAAGAATGGGACAGTGACTGACTTGAAGTTCCGCATCAAGTAAGACGAGATACGCAGAATCTCATGCCCTGTCCTCA
CCCTCAAAGGCCCAGTATACACTAGAGGGTCAGAATACTTAGCCACCAGTGCGGCAGGATCCCTTGAAACAGGGATTCCCCTTTTGTGAGAACCTTTGAA
CTGGTACCTAGGTGCAACCAATGAAAAAATTGGTGCCACAGCCTGCATTTCGATAACAAACACAAAGTTAATGAAAGAAGACACTGCTGTGAAAACACAT
TCATCAAAGCACACGAAAATAACCATATAAGGGTTAGGCGCCTTACACTAACA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.963905
Expected error %
0.251208
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g11650.1 Score 233 E Value 9e-62 Identity 26.80% Align. Length 410bp (62.79%) Frame 1
68418.m01362 hydrolase, alpha/beta fold family protein contains Pfam profile PF00561: hydrolase, alpha/beta fold family; low similarity to monoglyceride lipase from [Homo sapiens] GI:14594904, [Mus musculus] GI:2632162
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g73480.1 Score 177 E Value 6e-45 Identity 20.70% Align. Length 395bp (60.49%) Frame 1
68414.m08507 hydrolase, alpha/beta fold family protein low similarity to monoglyceride lipase from [Homo sapiens] GI:14594904, [Mus musculus] GI:2632162; contains Pfam profile PF00561: hydrolase, alpha/beta fold family
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_913160.1| Score 233 E Value 4e-60 Identity 27.60% Align. Length 398bp (60.95%) Frame 1
lysophospholipase - like [Oryza sativa (japonica cultivar-group)] dbj|BAB89422.1| monoglyceride lipase isoform 2 -like [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB87683.1| Score 233 E Value 4e-60 Identity 26.80% Align. Length 410bp (62.79%) Frame 1
lysophospholipase-like protein [Arabidopsis thaliana] ref|NP_196726.1| hydrolase, alpha/beta fold family protein [Arabidopsis thaliana] pir||T48524 lysophospholipase-like protein - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0140G03
Clone
Clone name
hba-140g3
LE_HBa0140G03
C08HBa0140G03
P140G03
Clone type
BAC
Contamination
none
Estimated length
99,219
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found