Clone Read LE_HBa0053C22_T7_43531

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0053C22_T7_43531
Primer
T7
ID from Seq. Facility
LE_HBa_053_C22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
748 trimmed for vector and quality, 896 raw
Sequence
>LE_HBa0053C22_T7_43531 (raw untrimmed)
GAACCCTGGGCCCGTGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATAAACAAAATATCGAAATATCATAATCTCTTCATGCTCATCGCTGA
GCATGACTCTCCTTTATAGAAAGTATAAACATATCTTTAAATAATTTGACATAATTTATAGTGATGATCTAAAATAACATATCAAAACACTTATTTAAAA
TCATAACATCATATCATAAATTTTCGTTTTAAAAAGAATATTTTTGTTCTTTTTTTAGTTTGTTTTAAAAAAAATGATCTCTTTCCTTTTTTGCTGACAT
TTTAACTTTAACTTTCCACGTAACATGTTTAAGATCACAAAATTAAAGGATATTGTGATAAATTTAACATAACTTTAGTTTAGAACCACAAATTAAAAAA
TCTTTTTTCTTTTCTTAAATTCCGTTCTAAATCAAACTATTCATTTTACAGTATAAATTTAAAATATTTTCATTGGTTTATCTCTTGTCCACAAGCCATT
AATAATAATAGAGGAATTAAAGAGCGTTGAATTTTGACATCATTAATAGAAACAAGTCAGTAGATTATTATTTAATAGAAGCAATGCGTTTTCGATATAT
CATTGGTTCGTACCATTTTTGACGTCTTTCAAACATGTGTACCACCTTTAATTTCCATTTTTATGGACTATACTTTCTTTACTCAATTCTTTTTTTAATA
TAAAAAAATAGTAAATAAATATTTGATACTCTTTTTTTTTTTTTTTTTTCTGTTTTCAGTTAGCTTTTTAGGATTACCAAAACTAGTTAAGGATGAGGGT
TTTGTTACTACCTACCATTCCAATTATATTATTCGTTGAAATCTCTTTTTTTTTTTTTCCCTTGTGGGGGTTATGAAAAGAACTTTAAGAAATTAA
>LE_HBa0053C22_T7_43531 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTATAAACAAAATATCGAAATATCATAATCTCTTCATGCTCATCGCTGAGCATGACTCTCCTTTATAGAAA
GTATAAACATATCTTTAAATAATTTGACATAATTTATAGTGATGATCTAAAATAACATATCAAAACACTTATTTAAAATCATAACATCATATCATAAATT
TTCGTTTTAAAAAGAATATTTTTGTTCTTTTTTTAGTTTGTTTTAAAAAAAATGATCTCTTTCCTTTTTTGCTGACATTTTAACTTTAACTTTCCACGTA
ACATGTTTAAGATCACAAAATTAAAGGATATTGTGATAAATTTAACATAACTTTAGTTTAGAACCACAAATTAAAAAATCTTTTTTCTTTTCTTAAATTC
CGTTCTAAATCAAACTATTCATTTTACAGTATAAATTTAAAATATTTTCATTGGTTTATCTCTTGTCCACAAGCCATTAATAATAATAGAGGAATTAAAG
AGCGTTGAATTTTGACATCATTAATAGAAACAAGTCAGTAGATTATTATTTAATAGAAGCAATGCGTTTTCGATATATCATTGGTTCGTACCATTTTTGA
CGTCTTTCAAACATGTGTACCACCTTTAATTTCCATTTTTATGGACTATACTTTCTTTACTCAATTCTTTTTTTAATATAAAAAAATAGTAAATAAATAT
TTGATACTCTTTTTTTTTTTTTTTTTTCTGTTTTCAGTTAGCTTTTTA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.883479
Expected error %
0.305466
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L38948.1:25-452 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.28%) Frame 2
Cloning vector pKIL128 ccdB gene, complete cds
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13871.1:182-319 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.28%) Frame 2
pT7T3D cloning vector
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0053C22
Clone
Clone name
hba-53c22
LE_HBa0053C22
C12HBa0053C22
P053C22
Clone type
BAC
Contamination
none
Estimated length
60,533
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found