Clone Read LE_HBa0009L09_T7_4385

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0009L09_T7_4385
Primer
T7
ID from Seq. Facility
LE_HBa_009_L09-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
51 trimmed for vector and quality, 902 raw
Sequence
>LE_HBa0009L09_T7_4385 (raw untrimmed)
TAACTGGGTCCGAGAGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGCTACTGTTTAATTTCTTCTGGCCTTAAGAGGCACNANANNNGCNNCNCN
ANGNNNGGNCNGAACTTGAAACGGGGNANANNNNCAAGCACNNCACAAACCAANGANAANANAACNGNGNGCNCAAAGGACGNGAACCNCGCCGNNNNGG
ANNGNNGCNGANCANNNNAGAANNNGCCGCANGNGNAGCAGNACNAGNNCANGNGCNNNAANACCAGNNNGANANNGNNNGGAANCANNCNCGANNNGCN
GGNNCNNGGAANNCNACNGNNGGACNNCAGNACNGAANGGNAGGNNAGGGGNCANCCAANCNNNAAACNNCGANAGAACNGNNNCNNNNGCCNAGGGCAG
ACNAGGCACANCANCGNCNGGANACACNACAGAAGCCCAGNANNNAAGNGAAGAAGAGNAGNACAGCNACNGANNAAGAANNGNNNCACNNNCNNACNAN
GNNAGNCGNCGCANGCANGCNGCGANNCNNGANCNCNAGANNNCAAGAACCANNGNGAGAACNNCGNCNNGCCANCANAGCAACNNCAGGNGCANGNGCN
NGCCANNNNNGGGCGNCNNAGNNNNNCAAAGGGAAAACNAAGNCACANGNGGCGACNAAGNNANANCNCGAGANAACNAGNNNAAGGGCGAGNANNCNGG
GAAAACCAGNNCNCGACCAAGCGGACAAGCCAGGAANCCAAAAGCAAAGGNCGCCGCGGAAGAACCCAAAGGAAAGCCCCGNAAANAACACAAAAAAGGA
GGAAAACCCCCCCACCCCCACCAAGGGGAAAGGAACCCGGAAAAGGAAAAAAACCCCAAGAACACCCCCCCAACCCCAAGGACCCCCCCCGCCAAACGCC
CA
>LE_HBa0009L09_T7_4385 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTGCTACTGTTTAATTTCTTCT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
  • Short cloning insert
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.82977
Expected error %
0.163528
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L08951.1:26-123 Score 66 E Value 3e-13 Identity 100.00% Align. Length 32bp (62.75%) Frame 2
pT7T318 cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M62983.1:27-213 Score 66 E Value 3e-13 Identity 100.00% Align. Length 32bp (62.75%) Frame 2
Cloning vector M13JN72 containing mutliple cloning site
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0009L09
Clone
Clone name
hba-9l9
LE_HBa0009L09
P009L09
Clone type
BAC
Contamination
none
Estimated length
124,567
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found