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Clone Read LE_HBa0059L14_T7_47019

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0059L14_T7_47019
Primer
T7
ID from Seq. Facility
LE_HBa_059_L14-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
746 trimmed for vector and quality, 954 raw
Sequence
>LE_HBa0059L14_T7_47019 (raw untrimmed)
TTTGACTGGTACCGGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCACCCGTGTGCTCGACGTAGTTACAACAATTAGTCACTCAAGAATCTA
AATTTCTCGTGATACATATAGTGTTTACCCTAACTGTCAGCTAATGCCAGCCTCTGTTTATGAGTATCAGTTGGTAACTTGACATCAGTGGCCAATCCAA
TAGCTTGAAGAAATCGTACTACATACCAAGTCATGTCAAGTTGCCACCATTTTAATCCATGCCTGGCTGAATACTCAAACGCGTGGTGGTTGTTGTGCCA
ACCTTCTCCAAATGCAAGCAATGCCACCCACCTATATTATACAAAATTGAGTCCCCAAAAATGGACAAAAAGAAAAGAAGCAACAAGACAAATAAATTTT
AAAACTTTTATACTTACCAGTTGTTCCTTGATAAGTCACCAGTATTCCACGCCTGCTTTCCCCATACATGGCATGCTGAATTTACTAGCCAAGTTATGTG
GTATACCCACACAATTCTCACCCCCTGCACACGCAGTGAGTTTAGCAGTTGAATTTTCAGAATCTCATTGCACCAAATGCATAGAACTCAGAATTTTGCC
TATGTTACTATCGGATAATCACCCAAATACTAGTTTTAAGTTTGATTTCTGATAGCCATGCTGATCAATTCCTTAAAAGGTACTAGAACTATTTCTTTTA
CCCTCGAAGGACATCACTATCATCAAACATCAATCAATTGCCAGTGAGATCCAAAGGACATGTTGTATGGAACAGCAACAAATTAGCACTTGATTTTGTC
CAGTAATTCTTTAAGAAAACAAAGACCCTACAACTGATAACACAACAACTTTTCTTCACAAAATTGGCTTACACTTGATTTAGGCGGGAAAAAGATCCAA
AACATTAGGGATTTGAACCTGCCGAACCGTAATCTAAAAGGAAAAAGAAATTCG
>LE_HBa0059L14_T7_47019 (trimmed for quality and cloning vector)
AGCTTCACCCGTGTGCTCGACGTAGTTACAACAATTAGTCACTCAAGAATCTAAATTTCTCGTGATACATATAGTGTTTACCCTAACTGTCAGCTAATGC
CAGCCTCTGTTTATGAGTATCAGTTGGTAACTTGACATCAGTGGCCAATCCAATAGCTTGAAGAAATCGTACTACATACCAAGTCATGTCAAGTTGCCAC
CATTTTAATCCATGCCTGGCTGAATACTCAAACGCGTGGTGGTTGTTGTGCCAACCTTCTCCAAATGCAAGCAATGCCACCCACCTATATTATACAAAAT
TGAGTCCCCAAAAATGGACAAAAAGAAAAGAAGCAACAAGACAAATAAATTTTAAAACTTTTATACTTACCAGTTGTTCCTTGATAAGTCACCAGTATTC
CACGCCTGCTTTCCCCATACATGGCATGCTGAATTTACTAGCCAAGTTATGTGGTATACCCACACAATTCTCACCCCCTGCACACGCAGTGAGTTTAGCA
GTTGAATTTTCAGAATCTCATTGCACCAAATGCATAGAACTCAGAATTTTGCCTATGTTACTATCGGATAATCACCCAAATACTAGTTTTAAGTTTGATT
TCTGATAGCCATGCTGATCAATTCCTTAAAAGGTACTAGAACTATTTCTTTTACCCTCGAAGGACATCACTATCATCAAACATCAATCAATTGCCAGTGA
GATCCAAAGGACATGTTGTATGGAACAGCAACAAATTAGCACTTGA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.965133
Expected error %
0.295152
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g15850.1 Score 125 E Value 6e-49 Identity 85.50% Align. Length 379bp (50.80%) Frame 3
68416.m02005 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g15870.1 Score 110 E Value 8e-36 Identity 73.00% Align. Length 382bp (51.21%) Frame 3
68416.m02007 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAN41357.1| Score 125 E Value 3e-47 Identity 85.50% Align. Length 379bp (50.80%) Frame 3
putative delta 9 desaturase [Arabidopsis thaliana] dbj|BAB02316.1| delta 9 desaturase-like protein [Arabidopsis thaliana] sp|Q949X0|ADS3_ARATH Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplast precursor (Monogalactosyldiacylglycerol-specific palmitic acid desaturase) (Delta 7 desaturase) (FAD5) ref|NP_566529.1| fatty acid desaturase family protein [Arabidopsis thaliana] dbj|BAD23903.1| monogalactosyldiacylglycerol-specific palmitic acid desaturase [Arabidopsis thaliana] gb|AAW51920.1| palmitoyl-monogalactosyldiacylglycerol delta7-desaturase [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM63459.1| Score 125 E Value 3e-47 Identity 85.50% Align. Length 379bp (50.80%) Frame 3
putative delta 9 desaturase [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0059L14
Clone
Clone name
hba-59l14
LE_HBa0059L14
C03HBa0059L14
P059L14
Clone type
BAC
Contamination
none
Estimated length
66,329
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found