Clone Read LE_HBa0011N20_T7_5707

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0011N20_T7_5707
Primer
T7
ID from Seq. Facility
LE_HBa_011_N20-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
847 trimmed for vector and quality, 952 raw
Genbank Accession
DU025334
Sequence
>LE_HBa0011N20_T7_5707 (raw untrimmed)
AATATACTGGGACCCGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGATGAGCAAATGATAGTGTTGTGCCTTTCCCAATCCTTTTGTGAGCA
AGTCAACTGACTATTCTTTAGTTCCTATGTACTGAGTCTTGATTAATCCCTGCTGCATTTTATCCTTACAAAGTGACAGTCAATGTCTATATGTTTGGTT
CGCTCATGAAAAATTGAATTTGTTGCTATTTGAATTGCAGCCTTTCTATCACAATGCAGATTAGTAGGTTGCACAAGTGAGACACCTAACTCTTAAAAAA
GCTATGTCAGCCAAGATATTTCTGCAAGAGTGGATGCCATACTTCTGAATTCAGCTTCTGCAGAACTTCTCGAGAGAGTATCCTGCTTCTTTCACTTCCA
TGACACTAAGAAAGAACTAAATTTAACCATATAACCTGTGACTGATTTCATGGTCTATGGACAAGCTTCCCAGTCTGAGTCACAATATGCAGTTAAGCTA
TGAGATGATGCTGAAGACATAAGCATACCAAGTCCTGGGGAATTTTTTATGTACCTTATTACTCTAAGTGCAGCCAACATATGAGATTCTTTTGGCTTGT
GCATAAATTGACTAAGTAGTTGGACAGCATAGGCAATATCAGGTCTAGTCATAGTTAGGTATAGTAATCTTCCTATCAATCTTCAATAACTTATTGAATC
AGGTAAAAACTTGTCATTATCATTTCCTGTGGCTATATCAAACTCATAAAATGAGTCTTTGATTGAAATCAAGAGGAGTATAAACAGGTTTAGCTCCCCC
TTGCCCTGAATTAGCAATTATTTCAAGGGCATAATTCCTCTGATTTACCAAAATTCCAGAAGAGGACCTCGAAAATTCATTGCCAAGAAAAATTTAAGAG
CAACCTAATTCTTCATCCAGAACTTCTTCTGAAGTTAGTTCTGGTGGACTGA
>LE_HBa0011N20_T7_5707 (trimmed for quality and cloning vector)
AGCTTGATGAGCAAATGATAGTGTTGTGCCTTTCCCAATCCTTTTGTGAGCAAGTCAACTGACTATTCTTTAGTTCCTATGTACTGAGTCTTGATTAATC
CCTGCTGCATTTTATCCTTACAAAGTGACAGTCAATGTCTATATGTTTGGTTCGCTCATGAAAAATTGAATTTGTTGCTATTTGAATTGCAGCCTTTCTA
TCACAATGCAGATTAGTAGGTTGCACAAGTGAGACACCTAACTCTTAAAAAAGCTATGTCAGCCAAGATATTTCTGCAAGAGTGGATGCCATACTTCTGA
ATTCAGCTTCTGCAGAACTTCTCGAGAGAGTATCCTGCTTCTTTCACTTCCATGACACTAAGAAAGAACTAAATTTAACCATATAACCTGTGACTGATTT
CATGGTCTATGGACAAGCTTCCCAGTCTGAGTCACAATATGCAGTTAAGCTATGAGATGATGCTGAAGACATAAGCATACCAAGTCCTGGGGAATTTTTT
ATGTACCTTATTACTCTAAGTGCAGCCAACATATGAGATTCTTTTGGCTTGTGCATAAATTGACTAAGTAGTTGGACAGCATAGGCAATATCAGGTCTAG
TCATAGTTAGGTATAGTAATCTTCCTATCAATCTTCAATAACTTATTGAATCAGGTAAAAACTTGTCATTATCATTTCCTGTGGCTATATCAAACTCATA
AAATGAGTCTTTGATTGAAATCAAGAGGAGTATAAACAGGTTTAGCTCCCCCTTGCCCTGAATTAGCAATTATTTCAAGGGCATAATTCCTCTGATTTAC
CAAAATTCCAGAAGAGGACCTCGAAAATTCATTGCCAAGAAAAATTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.958284
Expected error %
0.771739
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23160.1 Score 171 E Value 5e-49 Identity 46.90% Align. Length 726bp (85.71%) Frame 3
68417.m03342 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU89728.1| Score 253 E Value 5e-82 Identity 60.80% Align. Length 839bp (99.06%) Frame 1
putative retroelement pol polyprotein-like [Solanum tuberosum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAA97287.1| Score 212 E Value 4e-67 Identity 54.00% Align. Length 839bp (99.06%) Frame 1
retroelement pol polyprotein-like [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0011N20
Clone
Clone name
hba-11n20
LE_HBa0011N20
P011N20
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found