Clone Read LE_HBa0136D24_SP6_69694

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0136D24_SP6_69694
Primer
SP6
ID from Seq. Facility
LE_HBa_136_D24-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
241 trimmed for vector and quality, 655 raw
Sequence
>LE_HBa0136D24_SP6_69694 (raw untrimmed)
CCCCACCTTTTTGGATAGGGGGTGCGAATAGCATTTATCTTCTTTGTATCTGCATCCTTATTGAATGACCAAATAATTCAAGGAAGGGCGGACTGCGAAG
AGGGTCCTTTTAAGTAGATGACTCGTCGGGCGGTCGGAGCGGGACTTTTTTGGGAAAAAGAACTAGAATAAGTTTGTTTTTCTAAGGGAGTCATCATTTT
CTACCAGGAATGCTATATCATTTTCAATCCTGGCGTATTTCAGAGGCTTGAAGGACCCGCTGCCATGAATTGATTTAGAAAGATTCTTCTTTATCGCTGG
TGATCGGTCTTGGTATCAATATCCTTGCTTCTTTCTCGGCCAGATCCCGATTTCGTTTTGCTTCTCTCGATCATTCAGTCTGATCAGCTCTAACATCTCC
CCCGCCCCCCGCCCTTCAACTCCTTTAGGTTTGCTTTTGTATCATCAATTCGACTATTCTATCCCAGACATCCCATCCTTCCCTCATCTTCCCCTCTTGT
ATTTTATTTCTTCTTTGTTTTGCTTTTTACCTCTCTTTTCCCTTTTCTCTTCCTTTTCCCTCGTGTTTATCTTCATTTTTGCCCATCTTGATTGTTTGTG
TTTCTTAATATACTGTCTTCTTTTATGTTTTTCGGCTTCTCTTACTTTTTATCAG
>LE_HBa0136D24_SP6_69694 (trimmed for quality and cloning vector)
GAATGACCAAATAATTCAAGGAAGGGCGGACTGCGAAGAGGGTCCTTTTAAGTAGATGACTCGTCGGGCGGTCGGAGCGGGACTTTTTTGGGAAAAAGAA
CTAGAATAAGTTTGTTTTTCTAAGGGAGTCATCATTTTCTACCAGGAATGCTATATCATTTTCAATCCTGGCGTATTTCAGAGGCTTGAAGGACCCGCTG
CCATGAATTGATTTAGAAAGATTCTTCTTTATCGCTGGTGA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.958165
Expected error %
0.636476
Sequence Annotations 
Automatic Annotations
Arabidopsis thaliana Mitochondria (screening/organelle/ATH1_mitochondria)NCBI BLAST
mitochondria Score 161 E Value 3e-41 Identity 83.60% Align. Length 231bp (95.85%) Frame 1
No sequence description found.
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA47420.1| Score 129 E Value 2e-29 Identity 27.50% Align. Length 239bp (99.17%) Frame 3
S3 [Petunia x hybrida] pir||S31847 ribosomal protein S3 - garden petunia mitochondrion sp|Q04716|RT03_PETHY Mitochondrial ribosomal protein S3
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD83474.1| Score 129 E Value 2e-29 Identity 27.50% Align. Length 239bp (99.17%) Frame 3
ribosomal protein S3 [Nicotiana tabacum] ref|YP_173410.1| ribosomal protein S3 [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0136D24
Clone
Clone name
hba-136d24
LE_HBa0136D24
P136D24
Clone type
BAC
Contamination
none
Estimated length
110,942
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found