Clone Read LE_HBa0033L11_T7_7077

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0033L11_T7_7077
Primer
T7
ID from Seq. Facility
LE_HBa_033_L11-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
762 trimmed for vector and quality, 927 raw
Genbank Accession
DU026448
Sequence
>LE_HBa0033L11_T7_7077 (raw untrimmed)
GTCAACCGGGTCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTAAAATTTTCCCATCTATGCATCCCTTTCACGATTAACTAAATAACA
TTGCAACATGCTGCTAACCTTTAGGGTTATATACTTTTAATAGCAAAGGAGAACAATATAAGGGAAAATAACATGAGACAGTTGCTGCTGTCAACATGAA
ACAGCAGGTTGTATTCTTGCAATGAGATGTTCCACTAATTCGGTTTATTTACCCCTTTTTGCTGAGCATTTCAGAATAAAATTTAACCTGTCTTTGAAAT
GGAAAAAAATATGTGCAGTTTGTTCATCTTTGGTGTTATGAACGCAACTGCATTGCTCTTCCATATATTTTTTTTTGATGAAGTAAAGTTGTATTCCTCA
GCAATGAGGGTATGCTAGCAACCATCAAAAAGATTTACATCAGTGACCCAAAAAAAAACCAACTCAAGAAATTGCTACTAAACTTATAAAGAAGAAAAAA
AGTTTAAAATCTGTCCAGAATCCTCTATACCCTTCTCATTACACCAAAAATAGGTCTTCATACAACACCATTTGATCTTTTTTACTGATTCTGCAGTATT
TTCCATAAACCTCTTGTTTCTTTCTCTCCAAATAGTCCACCATACAAGAGGGTATCATGTTCCACCATTTCTTCTGGCTCTTACTCCCCCCCTTCCATAT
TTTTATGTAGATAGGTAATAGCATAAATGTTGGCATGCATCTAAGTTAAAATATGTGCAGGTTCTTAAATTCTATACTTCTTAAGATGTACTCTGTTTTC
TGTTAATCACTGAACTAGCTTCTCAAAATTTCAAATATAAATAAATTCGTGCAATTGTTGCTGGACTTCTTATTGTTCCCAGGTCCGAAGGTGCACCGAC
TCAGAACCCAGAGAATACCAGAACAAA
>LE_HBa0033L11_T7_7077 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTAAAATTTTCCCATCTATGCATCCCTTTCACGATTAACTAAATAACATTGCAACATGCTGCTAACCTT
TAGGGTTATATACTTTTAATAGCAAAGGAGAACAATATAAGGGAAAATAACATGAGACAGTTGCTGCTGTCAACATGAAACAGCAGGTTGTATTCTTGCA
ATGAGATGTTCCACTAATTCGGTTTATTTACCCCTTTTTGCTGAGCATTTCAGAATAAAATTTAACCTGTCTTTGAAATGGAAAAAAATATGTGCAGTTT
GTTCATCTTTGGTGTTATGAACGCAACTGCATTGCTCTTCCATATATTTTTTTTTGATGAAGTAAAGTTGTATTCCTCAGCAATGAGGGTATGCTAGCAA
CCATCAAAAAGATTTACATCAGTGACCCAAAAAAAAACCAACTCAAGAAATTGCTACTAAACTTATAAAGAAGAAAAAAAGTTTAAAATCTGTCCAGAAT
CCTCTATACCCTTCTCATTACACCAAAAATAGGTCTTCATACAACACCATTTGATCTTTTTTACTGATTCTGCAGTATTTTCCATAAACCTCTTGTTTCT
TTCTCTCCAAATAGTCCACCATACAAGAGGGTATCATGTTCCACCATTTCTTCTGGCTCTTACTCCCCCCCTTCCATATTTTTATGTAGATAGGTAATAG
CATAAATGTTGGCATGCATCTAAGTTAAAATATGTGCAGGTTCTTAAATTCTATACTTCTTA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.946383
Expected error %
0.325741
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.20%) Frame 2
Cloning vector pMAMneoBlue
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02437.1:4662-4674-49 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.20%) Frame 2
Cloning vector pT3/T7-LUC
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0033L11
Clone
Clone name
hba-33l11
LE_HBa0033L11
C12HBa0033L11
P033L11
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found