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Clone Read LE_HBa0147J24_T7_73055

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0147J24_T7_73055
Primer
T7
ID from Seq. Facility
LE_HBa_147_J24-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
777 trimmed for vector and quality, 829 raw
Genbank Accession
DU065566
Sequence
>LE_HBa0147J24_T7_73055 (raw untrimmed)
GGCTAAACTGGGACCGCGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGATTTTTCAGAAGGAGAAGACCTCTTCAGCCTCTTATGTTTTTGA
TTTCTTGAGTCAGATGTTGAGTTCTTCAAAATTCTTGGAGAGCTATTAATGGCCCTATGCAACGTCCGAGCTAACTCTTCATCTGTTCTACAATCATCAG
TCCCCTTATTCTTATTATACAGCGTCTGAACAGGAACATGTTTTTTCATCTTATTCCTCTTCAAATGTTTGTCCCTACTGGTTCCCTCATCAGAGAGAGT
GGCAACCAATTCTAGAGCGCTCTTAGCAGCAGCCACAGCCTTTGCTGCTTTCGCAGCCTTCTCTTCTGCAATGGCTCTTGCAGCTTTTGCAGCCTTCACA
GCTTCTAGAGCTGCCAATCTTGAGGCCTCAGCAATCTCCTCATTAGATCGATTAGAGTCTCCAAAATGAGACTTCAATAGATTATTAAGACGGATATCAA
AATCACACGGGGGACTATTAACAGTTGGTGGGGATGGGGTAGATGAGTAAACATCAGAATTCTGCTTCTTCAATCCAGCAAGAAGCCGCTTACGTGGAGG
CAAAAGCACATCGGCATCCAAGTGATTAATATCACATACATTAGCTTCCATCTTTTTCCTACATCTACACACTTTCAAACTACAAAAACAACATTAAAAA
TCACACCTTTATTAGCACAAAGTTTTATAAAAAACAAAACTACCAGCTGACTGCTTGTTATTTCCAAACCCTACATTAGATTCACATACTATTAACCCTA
AACTACCAAAACTCAATCGCTCCATTCAT
>LE_HBa0147J24_T7_73055 (trimmed for quality and cloning vector)
AGCTTAGATTTTTCAGAAGGAGAAGACCTCTTCAGCCTCTTATGTTTTTGATTTCTTGAGTCAGATGTTGAGTTCTTCAAAATTCTTGGAGAGCTATTAA
TGGCCCTATGCAACGTCCGAGCTAACTCTTCATCTGTTCTACAATCATCAGTCCCCTTATTCTTATTATACAGCGTCTGAACAGGAACATGTTTTTTCAT
CTTATTCCTCTTCAAATGTTTGTCCCTACTGGTTCCCTCATCAGAGAGAGTGGCAACCAATTCTAGAGCGCTCTTAGCAGCAGCCACAGCCTTTGCTGCT
TTCGCAGCCTTCTCTTCTGCAATGGCTCTTGCAGCTTTTGCAGCCTTCACAGCTTCTAGAGCTGCCAATCTTGAGGCCTCAGCAATCTCCTCATTAGATC
GATTAGAGTCTCCAAAATGAGACTTCAATAGATTATTAAGACGGATATCAAAATCACACGGGGGACTATTAACAGTTGGTGGGGATGGGGTAGATGAGTA
AACATCAGAATTCTGCTTCTTCAATCCAGCAAGAAGCCGCTTACGTGGAGGCAAAAGCACATCGGCATCCAAGTGATTAATATCACATACATTAGCTTCC
ATCTTTTTCCTACATCTACACACTTTCAAACTACAAAAACAACATTAAAAATCACACCTTTATTAGCACAAAGTTTTATAAAAAACAAAACTACCAGCTG
ACTGCTTGTTATTTCCAAACCCTACATTAGATTCACATACTATTAACCCTAAACTACCAAAACTCAATCGCTCCATT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.965098
Expected error %
0.294012
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g35510.1 Score 91 E Value 7e-19 Identity 10.70% Align. Length 578bp (74.39%) Frame 1
68417.m05048 expressed protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM67065.1| Score 91 E Value 3e-17 Identity 10.70% Align. Length 578bp (74.39%) Frame 1
unknown [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAB80267.1| Score 91 E Value 3e-17 Identity 10.70% Align. Length 578bp (74.39%) Frame 1
putative protein [Arabidopsis thaliana] emb|CAA20021.1| putative protein [Arabidopsis thaliana] ref|NP_195276.3| expressed protein [Arabidopsis thaliana] pir||T04656 hypothetical protein F8D20.20 - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0147J24
Clone
Clone name
hba-147j24
LE_HBa0147J24
P147J24
Clone type
BAC
Contamination
none
Estimated length
95,945
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found