Clone Read LE_HBa0047H01_T7_9169

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0047H01_T7_9169
Primer
T7
ID from Seq. Facility
LE_HBa_047_H01-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
825 trimmed for vector and quality, 915 raw
Genbank Accession
DU028299
Sequence
>LE_HBa0047H01_T7_9169 (raw untrimmed)
GCGAACGGGGGTCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTGCTAACTTTAAGATCAACGTTTCTCATACTGAGTCTTCATTTGTTC
TTCCTTTTTGCTTTGACGCATGCTTCATCAATTATCAACAATCATTGCTTTTCTTATGCTGTTTGTTTCGTTTTCCTCCTCCTCCTTTAGGCTATTGGCG
AGTTCAACAATGCCCTACATGGAAATAAATCTGCAAAATGCAAACTTATTGTTTCTTCTCACAACTATGAAAGCACACCGTCAGCTGAGGATCTTGGCAA
TCTAGTAGCAAGAATACAGGCATCTGGAGCTGACATTGTGAAATTTGCTACAACTGCACAGGATATCACTGATGTTGCACGTGTTTTCCAAATTACTGTT
CATTCTCAAGTAAGAGGCGTAAGTAGTATAGTCAAGTTGTATAAGTTGTGTTAAAATATGCATGGTGATCCATCATGGTTTTTCCTCAGCTGGATTTGGT
GCATAATAATAGTAGCTGCTTGACTGGAATCTAGAAGCTGGCTGCTTTTTGCTTTTTGTATTAACTATTAAGCCATACATGCCAATAAGTTAGGTATAGC
AGCACAAACTTATGGCTGAGAATAACTTATTAAAAAATAAGTAATCGAGAATAACTTCCAAATTAACTCTCTCTCTCTCCCGGTGAAAATGGTGGGGGTT
TGTTAAATTGTCTTAATTTATCTGATGATTTTTTCCATTGCAAGAAAAATTTGAATGTAGATAGTAGAATTGATGCCTTGGAGACATTTTATCAAATGAA
TGGCATCCTGATATCACTTGTCAATTAAGGGGCCTTTTAGTACATCATGCGATTAGGATCGTGTTATATGCTGTTGGGTTGTTGGATTCTAGTTAAGGCT
GGTGGATTCTTAATG
>LE_HBa0047H01_T7_9169 (trimmed for quality and cloning vector)
CTAGAGTCGACCTGCAGGCATGCAAGCTTTGCTAACTTTAAGATCAACGTTTCTCATACTGAGTCTTCATTTGTTCTTCCTTTTTGCTTTGACGCATGCT
TCATCAATTATCAACAATCATTGCTTTTCTTATGCTGTTTGTTTCGTTTTCCTCCTCCTCCTTTAGGCTATTGGCGAGTTCAACAATGCCCTACATGGAA
ATAAATCTGCAAAATGCAAACTTATTGTTTCTTCTCACAACTATGAAAGCACACCGTCAGCTGAGGATCTTGGCAATCTAGTAGCAAGAATACAGGCATC
TGGAGCTGACATTGTGAAATTTGCTACAACTGCACAGGATATCACTGATGTTGCACGTGTTTTCCAAATTACTGTTCATTCTCAAGTAAGAGGCGTAAGT
AGTATAGTCAAGTTGTATAAGTTGTGTTAAAATATGCATGGTGATCCATCATGGTTTTTCCTCAGCTGGATTTGGTGCATAATAATAGTAGCTGCTTGAC
TGGAATCTAGAAGCTGGCTGCTTTTTGCTTTTTGTATTAACTATTAAGCCATACATGCCAATAAGTTAGGTATAGCAGCACAAACTTATGGCTGAGAATA
ACTTATTAAAAAATAAGTAATCGAGAATAACTTCCAAATTAACTCTCTCTCTCTCCCGGTGAAAATGGTGGGGGTTTGTTAAATTGTCTTAATTTATCTG
ATGATTTTTTCCATTGCAAGAAAAATTTGAATGTAGATAGTAGAATTGATGCCTTGGAGACATTTTATCAAATGAATGGCATCCTGATATCACTTGTCAA
TTAAGGGGCCTTTTAGTACATCATG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.955495
Expected error %
0.163724
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g06350.1 Score 91 E Value 1e-18 Identity 19.70% Align. Length 233bp (28.24%) Frame 3
68416.m00733 dehydroquinate dehydratase, putative / shikimate dehydrogenase, putative similar to dehydroquinate dehydratase/shikimate dehydrogenase [Nicotiana tabacum][GI:535771], dehydroquinate dehydratase/shikimate:NADP oxidoreductase [Lycopersicon esculentum][GI:3169883]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAC17991.1| Score 147 E Value 4e-34 Identity 30.50% Align. Length 242bp (29.33%) Frame 3
dehydroquinate dehydratase/shikimate:NADP oxidoreductase [Lycopersicon esculentum] gb|AAC17993.1| dehydroquinate dehydratase/shikimate:NADP oxidoreductase [Lycopersicon esculentum] pir||T06264 3-dehydroquinate dehydratase (EC 4.2.1.10) / shikimate 5-dehydrogenase (EC 1.1.1.25) - tomato
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAS90325.1| Score 130 E Value 3e-29 Identity 26.70% Align. Length 242bp (29.33%) Frame 3
3-dehydroquinate dehydratase / shikimate dehydrogenase isoform 1 [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0047H01
Clone
Clone name
hba-47h1
LE_HBa0047H01
P047H01
Clone type
BAC
Contamination
none
Estimated length
86,772
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found