Clone hba-128j14

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Clone
Clone name
hba-128j14
LE_HBa0128J14
C02HBa0128J14
P128J14
Clone type
BAC
Contamination
none
Estimated length
26,829
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1368Tomato-EXPEN 2000plate 7G12
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 2
FISH map 
View on FISH map chromosome 1
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
477-04 1CompositeFluorescencePhase95.7%
477-07 1CompositeFluorescencePhase94.7%
477-10 1CompositeFluorescencePhase94.6%
477-11 1CompositeFluorescencePhase96.9%
477-14 1CompositeFluorescencePhase95.6%
477-15 1CompositeFluorescencePhase95.4%
477-17 1CompositeFluorescencePhase95.0%
477-18 1CompositeFluorescencePhase93.5%
477-27 1CompositeFluorescencePhase95.8%
Mean±sd95.2%±1.0%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 19.1µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 2 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 2
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
45,004 bp
C02HBa0128J14.3
AC239447.3 (GenBank) 		[View]
End Sequences
Warnings
  • sequence length 45,004 is 67.7% different from estimated length 26,829
 FTP
Chromosome 2 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (7)
1975, 2425, 3586, 3804, 4012, 4205, 6822
HindIII in silico (10)
1360, 1970, 2456, 3636, 3863, 4014, 4252, 6858, 6869, 7436
Fragment Lengths Match Score
0.82
Annotations  
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LE_HBa0128J14 has no browsable sequence annotations
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Seq. Center Reported Status
Estimated Length
 and  bp
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Sequencing on Chromosome
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Annotations
End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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