Clone Read LE_HBa0128J14_T7_69195

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0128J14_T7_69195
Primer
T7
ID from Seq. Facility
LE_HBa_128_J14-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
747 trimmed for vector and quality, 887 raw
Genbank Accession
DU074209
Sequence
>LE_HBa0128J14_T7_69195 (raw untrimmed)
ATTAACCGGGTCCCGCGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGATGAATCTGGAATGCTGGATTGTCTCCAGTTATTGCATTTTCACAT
TGGATCTCAGATCCCCACAACAGAGTTGCTTGCTGATGGTGTTGGTGAGGCCACTCAGATTTACTCTGAATTAGTCCGTCTTGGAGCTGGTATGAAATTC
ATTGATATCGGAGGGGGGCTTGGAATCGACTATGACGGTTCTAAATCAAGCAATTCTGATGTCTCTGTTTGCTATAGCATTGAAGAATATGCCTCTGCTG
TTGTCCAAGCGGTCCTCTATGTCTGTGATCGTAAGGGCGTAAAGCATCCAGTGATTTGCAGCGAAAGTGGCAGGGCAATTGTTTCTCACCATTCAATTCT
GATTTTTGAAGCCGTGTCTGCTTCTACTAGTCATGTTTCTACACAGCCATCTTCGGGTGGTTTACAATCCTTGGTGGAGACTCTCAATGAAGATGCCCGT
GCTGACTACAGAAACTTATCTGCTGCTGCTGTCCGTGGAGAATATGATACATGTCTCATCTATTCTGATCAGTTGAAACAGAGATGTGTTGAACAGTTCA
AAGATGGGTCCTTGGATATTGAGCAGCTCGCTGCAGTGGATAGCATTTGTGATTGGGTGTCGAAGGCTATCGGGGTTGCTGATCCTGTCCGCACTTACCA
TGTGAATCTGTCAGTTTTCACCTCAATCCCTGATTTTTGGGGGCTCAGCCAATTGTTTCCTATTGTTCCAATTTCCCGTCTGGATGAAAAGCCTACAATG
AAAGGAATACTGTCTGACCTGACGTGTGACAGTGAAGGAAAGGTTGATAAGTTCTTGGGGGCGAATAAAGCTGCCCCTCCATGAAAT
>LE_HBa0128J14_T7_69195 (trimmed for quality and cloning vector)
CTAGAGTCGACCTGCAGGCATGCAAGCTTGATGAATCTGGAATGCTGGATTGTCTCCAGTTATTGCATTTTCACATTGGATCTCAGATCCCCACAACAGA
GTTGCTTGCTGATGGTGTTGGTGAGGCCACTCAGATTTACTCTGAATTAGTCCGTCTTGGAGCTGGTATGAAATTCATTGATATCGGAGGGGGGCTTGGA
ATCGACTATGACGGTTCTAAATCAAGCAATTCTGATGTCTCTGTTTGCTATAGCATTGAAGAATATGCCTCTGCTGTTGTCCAAGCGGTCCTCTATGTCT
GTGATCGTAAGGGCGTAAAGCATCCAGTGATTTGCAGCGAAAGTGGCAGGGCAATTGTTTCTCACCATTCAATTCTGATTTTTGAAGCCGTGTCTGCTTC
TACTAGTCATGTTTCTACACAGCCATCTTCGGGTGGTTTACAATCCTTGGTGGAGACTCTCAATGAAGATGCCCGTGCTGACTACAGAAACTTATCTGCT
GCTGCTGTCCGTGGAGAATATGATACATGTCTCATCTATTCTGATCAGTTGAAACAGAGATGTGTTGAACAGTTCAAAGATGGGTCCTTGGATATTGAGC
AGCTCGCTGCAGTGGATAGCATTTGTGATTGGGTGTCGAAGGCTATCGGGGTTGCTGATCCTGTCCGCACTTACCATGTGAATCTGTCAGTTTTCACCTC
AATCCCTGATTTTTGGGGGCTCAGCCAATTGTTTCCTATTGTTCCAA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.974751
Expected error %
0.344484
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65306.2:1-103 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.02%) Frame 2
Cloning vector pGEM-3Zf(+)
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g16500.1 Score 315 E Value 2e-86 Identity 21.90% Align. Length 722bp (96.65%) Frame 1
68415.m01892 arginine decarboxylase 1 (SPE1) (ARGDC) identical to SP|Q9SI64 Arginine decarboxylase 1 (EC 4.1.1.19) (ARGDC 1) (ADC 1) (ADC-O) {Arabidopsis thaliana}
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g34710.2 Score 312 E Value 1e-85 Identity 22.00% Align. Length 722bp (96.65%) Frame 1
68417.m04927 arginine decarboxylase 2 (SPE2) identical to SP|O23141 Arginine decarboxylase 2 (EC 4.1.1.19) (ARGDC 2) (ADC 2) (ADC-N) {Arabidopsis thaliana}
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||JQ2341 Score 444 E Value 1e-123 Identity 31.30% Align. Length 722bp (96.65%) Frame 1
arginine decarboxylase (EC 4.1.1.19) - tomato gb|AAA61347.1| arginine decarboxylase sp|P49726|SPE1_LYCES Arginine decarboxylase (ARGDC) (ADC)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAN77734.1| Score 406 E Value 1e-112 Identity 28.80% Align. Length 722bp (96.65%) Frame 1
arginine decarboxylase [Capsicum annuum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0128J14
Clone
Clone name
hba-128j14
LE_HBa0128J14
C02HBa0128J14
P128J14
Clone type
BAC
Contamination
none
Estimated length
26,829
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found